EndoTreat Endotoxin Sample Treatment Kit
$299.00
Endotoxin is a common contaminant found in samples containing recombinant proteins or nucleic acids purified from gram-negative bacteria such as E. coli. Endotoxins, also known as lipopolysaccharides (LPS), are heat-stable molecules associated with the outer membranes of certain gram-negative bacteria. Sub-nanogram levels of endotoxin can trigger immune responses and septic shock in mammals. Endotoxin also affects a number of cell types in tissue culture experiments. The Food and Drug Administration (FDA) has established strict guidelines for the allowable endotoxin content of injectable drugs. Given the risks of endotoxin contamination, accurate endotoxin detection and quantitation in protein/peptide samples is crucial. Currently, LAL and recombinant Factor C assays are the main methods of endotoxin testing. These assays rely on enzymatic reactions and are susceptible to factors that can inhibit or enhance enzymatic performance. Â
EndoTreat Sample Treatment Kit is an endotoxin removal and neutralization kit designed to eliminate the inhibitory effects of proteins and peptides on endotoxin detection assays. Formerly known as EndoBind and EndoPrep, EndoTreat is now exclusively available from Stable Biologics.
Endotoxin (lipopolysaccharide/LPS) is a common contaminant in recombinant proteins and nucleic acids purified from gram-negative bacteria such as E. coli. Many cationic proteins and peptides bind to endotoxin and mask its activity, causing inaccurate detection in LAL assays and recombinant Factor C (rFC) assays. Sub-nanogram levels of endotoxin can trigger immune responses and septic shock, making accurate endotoxin detection critical for pharmaceutical analytical testing and injectable drug manufacturing.
EndoTreat contains a proprietary protease solution that digests interfering proteins and peptides, enabling accurate endotoxin detection and quantification. It is chemically mild and fully compatible with major endotoxin detection assays.
Key Benefits:
- Compatible with LAL and recombinant Factor C endotoxin detection assays
- Applicable to peptides, proteins, and blood samples
- Quick and easy protocol — results in under 2 hours
- One kit supports at least 20 reactions
- Meets FDA guidelines for injectable drug endotoxin testing
Kit Contents:
- Sample Buffer
- Protease Mix
- Instruction Manual
- MSDS
EndoTreat is essential for pharmaceutical analytical testing labs, protein expression and purification workflows, and researchers working with recombinant proteins who need accurate endotoxin quantification.
